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lcfA [2017-8-11 16:0:3]
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lcfA [2017-8-11 16:0:3]

long chain acyl-CoA synthetase, involved in surfactin production
Locus
BSU28560
Isoelectric point
6.12
Molecular weight
62.52 kDa
Protein length
560 aa Sequence Blast
Gene length
1680 bp Sequence Blast
Function
fatty acid degradation
Product
long chain acyl-CoA synthetase
Essential
no
E.C.
6.2.1.3
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids

    • Gene

    Coordinates
    2,918,646 → 2,920,328

    The protein

    Catalyzed reaction/ biological activity

  • ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed

    • Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • LcfB, YhfT, YngI

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    lcfA-fadR-fadB-etfB-etfA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed
  • expression of the operon is strongly induced during biofilm formation PubMed
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    Biological materials

    Mutant

  • available in Mohamed Marahiel's lab PubMed
  • 1A852 ( lcfA::cat), PubMed, available at BGSC

    • References

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